Mapping and quantification of phosphorylation sites on protein kinase Akt by LC-MS/MS

ABSTRACT: Mass spectrometry analysis of various Akt species was a cornerstone for elucidating the mechanism of its activation/autoinhibition. By combining intact mass measurements with LC-MS/MS we were able to characterize Akt species comprehensively and relate their biological activity to specific phosphorylation patterns.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Markus Hartl  

LAB HEAD: Thomas A. Leonard

PROVIDER: PXD022044 | Pride | 2021-08-13

REPOSITORIES: Pride