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Tandem mass spectrometry analysis of the thiol proteome from Bacillus cereus grown in anaerobiosisTandem mass spectrometry analysis of the thiol proteome from Bacillus cereus grown in anaerobiosis

ABSTRACT: Tandem mass spectrometry analysis of Bacillus cereus samples grown in anaerobiosis for identifying the redox state of the thiols.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Bacillus Cereus (strain Atcc 14579 / Dsm 31)

SUBMITTER: Jean ARMENGAUD

LAB HEAD: Jean Armengaud

PROVIDER: PXD022049 | Pride | 2022-02-16

REPOSITORIES: Pride

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Redox proteomic study of Bacillus cereus thiol proteome during fermentative anaerobic growth.

Hamitouche Fella F Gaillard Jean-Charles JC Schmitt Philippe P Armengaud Jean J Duport Catherine C Dedieu Luc L

BMC genomics 20210907 1

<h4>Background</h4>Bacillus cereus is a notorious foodborne pathogen, which can grow under anoxic conditions. Anoxic growth is supported by endogenous redox metabolism, for which the thiol redox proteome serves as an interface. Here, we studied the cysteine (Cys) proteome dynamics of B. cereus ATCC 14579 cells grown under fermentative anoxic conditions. We used a quantitative thiol trapping method combined with proteomics profiling.<h4>Results</h4>In total, we identified 153 reactive Cys residue ...[more]

PMID: 34493209

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