The Akirin2 regulome and interactome reveal gene/protein targets, candidate functional complements and role in epigenetic regulation

ABSTRACT: The Akirin (AKR) family of transcription cofactors are involved in the regulation of different biological processes such as immunity, interdigital regression, muscle and neural development throughout the metazoan. This model regulatory proteins do not have catalytic or DNA-binding capability and exert its regulatory function primarily through interacting proteins such as transcription factors, chromatin remodelers, and RNA-associated proteins. The application of regulomics and interactomics to key transcriptional regulators such as AKR is important to better understand their role in cell biology and epigenetic regulation of gene expression. In this study, we focused on the human AKR2 regulome and interactome to advance the knowledge on the function of this highly conserved regulatory cofactor. Based on the key function of AKR2 in cell interactome, regulome and epigenetic gene regulation, herein we hypothesized that metazoan evolved to have AKR2 functional complements and different mechanisms for AKR2-mediated epigenetic regulation of gene expression. To address this hypothesis, experiments were conducted to identify AKR2 gene/protein targets, functional complements after akr2 knockdown in human cells and to provide evidence of different mechanisms that may be involved in AKR2-mediated chromatin remodeling. The results revealed multiple AKR2 gene/protein targets, identified immune response genes as candidate AKR2 functional complements and showed that AKR2 is not only a link with chromatin remodelers, but also directly interacts with histone H3, thus providing new information on the function of this protein

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Margarita Villar  

LAB HEAD: Margarita Villar

PROVIDER: PXD022073 | Pride | 2021-09-09

REPOSITORIES: Pride