Proteomics

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Immunopeptidomics by use of a microfluidics chip.

ABSTRACT: A microfluidics technology was implemented to the immunoaffinity purification process of MHC peptides in Ligandomics/Immunopeptidomics. The thus purified HLA peptides were analysed by LCMS with the nanoElute LC and TimsTOF Pro Mass Spectrometer from Bruker. The aim of the microfluidics implementation was to improve the sensitivity and robustness while also reducing antibody and other material requirements in the immunoaffinity purification protocol.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Ovary, B Cell, Bladder, Kidney

DISEASE(S): Ovarian Cancer,Urinary Bladder Cancer,Renal Cell Carcinoma

SUBMITTER: Rui Branca  

LAB HEAD: Janne Lehtio

PROVIDER: PXD022194 | Pride | 2022-02-17

REPOSITORIES: Pride

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Publications

Identification of HLA class I ligands from the tumor surface (ligandome or immunopeptidome) is essential for designing T-cell mediated cancer therapeutic approaches. However, the sensitivity of the process for isolating MHC-I restricted tumor-specific peptides has been the major limiting factor for reliable tumor antigen characterization, making clear the need for technical improvement. Here, we describe our work from the fabrication and development of a microfluidic-based chip (PeptiCHIP) and i  ...[more]

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