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STALLION SPERM PROTEINS AFTER CRYOPRESERVATION

ABSTRACT: Using a split sample design 30 ejaculates from 10 different stallions were analyzed as fresh spermatozoa, and another aliquot from the same ejaculate was analyzed as a frozen thawed sample. The proteome was studied under both conditions using UHPLC/MS/MS and bioinformatic analysis conducted to identify discriminant variables between both conditions.

INSTRUMENT(S):

ORGANISM(S): Equus Caballus (horse)

TISSUE(S): Sperm

SUBMITTER: FERNANDO JUAN PEÑA VEGA

LAB HEAD: FERNANDO JUAN PEÑA VEGA

PROVIDER: PXD022236 | Pride | 2021-03-18

REPOSITORIES: Pride

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			Action	DRS
	SpecFeaturesMS1.tsv	Tabular
	p100.mzXML	Mzxml
	p101.mzXML	Mzxml
	p102.mzXML	Mzxml
	p103.mzXML	Mzxml

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Publications

In Stallion Spermatozoa, Superoxide Dismutase (Cu-Zn) (SOD1) and the Aldo-Keto-Reductase Family 1 Member b (AKR1B1) Are the Proteins Most Significantly Reduced by Cryopreservation.

Gaitskell-Phillips Gemma G Martín-Cano Francisco E FE Ortiz-Rodríguez José M JM Silva-Rodríguez Antonio A Gil Maria C MC Ortega-Ferrusola Cristina C Peña Fernando J FJ

Journal of proteome research 20210303 5

Although cryopreservation is widely used in animal breeding, the technique is still suboptimal. The population of spermatozoa surviving the procedure experiences changes attributed to alteration in their redox regulation. In order to expand our knowledge regarding this particular aspect, the proteome in fresh and frozen thawed aliquots of equine spermatozoa was studied to identify the proteins most severely affected by the procedure. If alteration of redox regulation is a major factor explaining ...[more]

PMID: 33656888

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