Proteomics

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Medicago truncatula Seed Protein Plasticity in Response to the Environment

ABSTRACT: Because extreme environmental events are expected to increase with climate change, identifying candidate genes for stabilizing the protein composition of legume seeds or optimizing it in a given environment becomes increasingly important. To elucidate the genetic determinants of seed protein plasticity, major seed proteins from 200 ecotypes of Medicago truncatula grown in four contrasted environments were quantified after one-dimensional electrophoresis. Major protein bands were excised from the seed protein profile of five genotypes to identify protein variants.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Medicago Truncatula (barrel Medic) (medicago Tribuloides)

TISSUE(S): Seed

SUBMITTER: Valerie LABAS  

LAB HEAD: Christine Le Signor

PROVIDER: PXD022244 | Pride | 2021-08-17

REPOSITORIES: Pride

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Publications

Genetic determinants of seed protein plasticity in response to the environment in Medicago truncatula.

Cartelier Kevin K   Aimé Delphine D   Ly Vu Joseph J   Combes-Soia Lucie L   Labas Valérie V   Prosperi Jean-Marie JM   Buitink Julia J   Gallardo Karine K   Le Signor Christine C  

The Plant journal : for cell and molecular biology 20210412 5

As the frequency of extreme environmental events is expected to increase with climate change, identifying candidate genes for stabilizing the protein composition of legume seeds or optimizing this in a given environment is increasingly important. To elucidate the genetic determinants of seed protein plasticity, major seed proteins from 200 ecotypes of Medicago truncatula grown in four contrasting environments were quantified after one-dimensional electrophoresis. The plasticity index of these pr  ...[more]

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