Dataset Information

Dehydration-induced mitochondrial proteome dynamics and defense response in rice

ABSTRACT: Water-deficit or dehydration greatly influences plant development and reduces crop productivity, particularly in rain-fed areas across the world. Despite extensive research over the past several decades, little is known about dehydration management of mitochondria and cellular energy metabolism. We, for the first time, mapped dehydration-induced global changes in protein expression of the mitochondrial landscape to dissect the molecular mechanism, which might enable crop species to survive. Toward this, four-week-old rice seedlings were subjected to progressive dehydration by withholding water, and the stress severity was assessed by physicochemical reactions and mitochondrial architecture. The comparative mitochondrial proteomics analysis led to the identification of an array of stress-responsive proteins, presumably involved in a variety of cellular functions that includes energy production, organelle protein transportation and ROS detoxification, among others. The proteomics profile demonstrated that the alteration of tricarboxylic acid cycle intermediates is crucial for fuelling ATP production. Collectively, our results demonstrate that the global regulation of mitochondrial proteins is linked to dehydration response, which would favour genetic manipulation of crop species for better adaptation.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Oryza Sativa (rice)

TISSUE(S): Plant Cell

SUBMITTER: TUSHAR KANTI MAITI

LAB HEAD: Tushar Kanti

PROVIDER: PXD022269 | Pride | 2023-09-16

REPOSITORIES: Pride

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Project description:Sporobolus stapfianus is a desiccation tolerant (DT) grass and a member of the Poaceae family alongside prominent crop and forage species. Despite the worldwide distribution of this family, most of its species are vulnerable to water loss within their vegetative tissues. As a DT species, S. stapfianus, could, therefore, serve as a model species for identifying the molecular changes that enabled such a rare occurrence of the DT phenotype in resurrection grasses and the implications of such a unique adaptation for agriculture. A comprehensive gene expression profiling was performed in plants subjected to a dehydration/rehydration cycle using NimbleGen microarrays hybridization method. Our results showed that most transcripts were high in the hydrated state of S. stapfianus leaf tissues and that minor dehydration (> 60% relative water content; RWC) did not induce most transcripts, but did repress photosynthetic activity, which show the importance of curtailing the production of toxic elements to the DT phenotype. It took a loss of 40% RWC for induction of most transcripts. This shows that this species is pre-equipped to deal with mild dehydration, but requires stress-induced activation mechanisms to prepare for desiccation. In agreement with our transcriptomic data, a global metabolic analysis conducted in this species has shown that most metabolites accumulate to their highest levels below 45% RWC, which suggests the importance of those late stages of dehydration in preparing resurrection grasses for desiccation and for early stages of rehydration. In most cases, a 12-h rehydration was sufficient to reinstate pre-stress expression levels, which shows that only partial damage occurred during drying. Unlike during dehydration, our metabolomic data profiled after rehydration showed that there is no clear correlation between gene expression and metabolic abundance, which suggests that most of the compounds that are produced during rehydration are quickly utilized in active metabolism of restoration. The findings of the paper show that S. stapfianus is primed to respond to mild dehydration, although severe dehydration require inducible response, with 40-30% RWC being the peak of gene expression. As for photosynthesis, this species cease its photosynthetic activity even at high water levels (85% RWC) alleviating the need to activate antioxidants activity during mild dehydration.

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Dehydration-induced mitochondrial proteome dynamics and defense response in rice

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