Multiplexed CuAAC-Suzuki-Miyaura for tandem activity-based chemoproteomic profiling

ABSTRACT: Suzuki-Miyauara cross-coupling has been used for mass-spectrometry-based chemoproteomics, including for target deconvolution and site of labeling studies. We also demonstrated that Suzuki-Miyauara cross coupling and CuAAC can be combined for dual target labeling for mass spectrometry-based proteomics. Furthermore, multiplexed CuAAC Suzuki-Miyaura chemoproteomics platform has been successfully applied to identify the protein targets of bifunctional cysteine- and lysine-reactive crosslinking probes.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Keriann Backus  

LAB HEAD: Keriann Backus

PROVIDER: PXD022279 | Pride | 2021-02-03

REPOSITORIES: Pride