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Quantitative proteomic analysis of four subtypes of M2 macrophages

ABSTRACT: In this study, we used mass spectrometry and label-free quantification (LFQ) to characterize the global proteomics of polarized (M1, M2a, M2b, M2c, and M2d) and unpolarized (M0) phenotypes of macrophages from human THP-1 monocytes. The results described the biological functions of the four M2 macrophages subtypes and provided available references for identifying M2 macrophages or subtypes of M2 macrophages.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood, Macrophage

DISEASE(S): Disease Free

SUBMITTER: Chen Ma

LAB HEAD: Shisheng Sun

PROVIDER: PXD022320 | Pride | 2022-08-12

REPOSITORIES: Pride

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Proteomic characterization of four subtypes of M2 macrophages derived from human THP-1 cells.

Li Pengfei P Ma Chen C Li Jing J You Shanshan S Dang Liuyi L Wu Jingyu J Hao Zhifang Z Li Jun J Zhi Yuan Y Chen Lin L Sun Shisheng S

Journal of Zhejiang University. Science. B 20220501 5

Macrophages are widely distributed immune cells that contribute to tissue homeostasis. Human THP-1 cells have been widely used in various macrophage-associated studies, especially those involving pro-inflammatory M1 and anti-inflammatory M2 phenotypes. However, the molecular characterization of four M2 subtypes (M2a, M2b, M2c, and M2d) derived from THP-1 has not been fully investigated. In this study, we systematically analyzed the protein expression profiles of human THP-1-derived macrophages ( ...[more]

PMID: 35557041

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