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Protein quantification of mouse inner medulla (IM) sections using MS based proteomics - WT vs. Dicer(AQP2Cre+) mice

ABSTRACT: In this project we investigated the global proteome alteration of dissected mouse inner medulla (IM) sections between the WT and the Dicer(AQP2Cre+) mice. TMT labeling strategy was used to obtain protein quantification information from LC-MS based proteomic analysis.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Kidney

SUBMITTER: Lei Cheng

LAB HEAD: Robert A. Fenton

PROVIDER: PXD022327 | Pride | 2021-05-05

REPOSITORIES: Pride

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Dysregulation of Principal Cell miRNAs Facilitates Epigenetic Regulation of AQP2 and Results in Nephrogenic Diabetes Insipidus.

Petrillo Federica F Iervolino Anna A Angrisano Tiziana T Jelen Sabina S Costanzo Vincenzo V D'Acierno Mariavittoria M Cheng Lei L Wu Qi Q Guerriero Ilaria I Mazzarella Maria Cristina MC De Falco Alfonso A D'Angelo Fulvio F Ceccarelli Michele M Caraglia Michele M Capasso Giovambattista G Fenton Robert A RA Trepiccione Francesco F

Journal of the American Society of Nephrology : JASN 20210316 6

<h4>Background</h4>MicroRNAs (miRNAs), formed by cleavage of pre-microRNA by the endoribonuclease Dicer, are critical modulators of cell function by post-transcriptionally regulating gene expression.<h4>Methods</h4>Selective ablation of Dicer in AQP2-expressing cells (Dicer<sup>AQP2Cre+</sup> mice) was used to investigate the role of miRNAs in the kidney collecting duct of mice.<h4>Results</h4>The mice had severe polyuria and nephrogenic diabetes insipidus, potentially due to greatly reduced AQP ...[more]

PMID: 33727367

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