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Secretome analysis of human mammary gland fibroblasts and breast cancer-associated fibroblasts

ABSTRACT: Cancer Associated Fibroblasts (CAF) are a dominant and critical cell type of the tumour microenvironment and can lead to breast cancer progression. TGF-β has been reported to influence fibroblast to myofibroblast activation, which is similar to cancer associated fibroblast phenotype. To understand the mechanism of TGF-β mediated CAF phenotype, we have performed a comparative proteomic analysis of conditioned media from CAF (isolated from breast cancer biopsy tissues) and normal mammary fibroblasts engineered to over-express TGF-β1. Liquid chromatography/ tandem mass spectrometry (LC ESI Q-TOF-MS/MS) assay of conditioned media and Venn analysis of the acquired data, revealed approximately 185 common proteins secreted by the three fibroblast types (CAF, normal mammary fibroblasts and TGF-β over expressing mammary fibroblasts). Among these, 12 proteins exclusively overlap between normal fibroblasts and CAF and 20 proteins exclusively overlap between CAF and TGF-β1 over-expressing fibroblasts. This analysis reveals interesting targets which may be important in activation phenotype of CAF and breast cancer progression.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Fibroblast

DISEASE(S): Breast Cancer

SUBMITTER: Armin Gandhi

LAB HEAD: Kondaiah Paturu

PROVIDER: PXD022374 | Pride | 2025-08-26

REPOSITORIES: Pride

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			Action	DRS
	CAF-1.mgf	Mgf
	CAF-1.mgf-pride.xml.gz	Mgf
	CAF-1.xlsx	Xlsx
	CAF-1.zip	Other
	CAF-2.mgf	Mgf

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