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Phosphorylation of Rif1 by Plk1

ABSTRACT: Polo-like kinase 1 (Plk1) is an important protein kinase for checkpoint recovery and adaptation in response to DNA damage and replication stress. However, although Plk1 is present in S phase, little is known about its localization and function during unperturbed DNA replication. Here used recombinant XRif1 C-terminal domain and recombinant XPlk1 in an in vitro phosphorylation assay followed by LC/MS/MS to identify which residues are phosphorylated by Plk1 on Rif1 CTD.

INSTRUMENT(S):

ORGANISM(S): Xenopus Tropicalis X Xenopus Laevis

TISSUE(S): Oocyte

SUBMITTER: Chiara guerrera

LAB HEAD: Chiara Guerrera

PROVIDER: PXD022410 | Pride | 2021-08-26

REPOSITORIES: Pride

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			Action	DRS
	200827_KaMA_PhosphoRif_5_neg_9426.raw	Raw
	200827_KaMA_PhosphoRif_6_reaction_9427.raw	Raw
	allSpectra.HCD.FTMS.iso_2.zip	Other
	checksum.txt	Txt
	txt.zip	Other

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