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Functional proteomics of membrane lipid domains after Brij 98 solubilization of uninduced and phenobarbital-induced rat liver microsomes: Defining the membrane localization of the P450 enzyme system

ABSTRACT: In this study, we use Brij 98 to determine the lipid domain distribution of the ER proteome in rat liver microsomes with emphasis on that of the P450 enzyme system. Because of the profound effects of phenobarbital treatment on protein expression and lipid biosynthesis, we will examine the liver ER proteomes from both uninduced and phenobarbital-treated rats in order to determine if protein and lipid variability influence the domain localization of proteins. To ascertain possible functional roles associated with membrane domain localization, we will apply G.O. analysis and Ingenuity Pathway Analysis (IPA) to the proteins from the identified membrane domains. The biological processes, molecular functions, and cellular pathways associated with the membrane lipid domains will be described, and the potential biological implications of these associations will be discussed.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Liver

SUBMITTER: Jessie Guidry

LAB HEAD: Jessie J Guidry

PROVIDER: PXD022460 | Pride | 2026-04-27

REPOSITORIES: Pride

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Proteomic and Bioinformatics Analysis of Membrane Lipid Domains after Brij 98 Solubilization of Uninduced and Phenobarbital-Induced Rat Liver Microsomes: Defining the Membrane Localization of the P450 Enzyme System.

Reed James R JR Guidry Jessie J JJ Backes Wayne L WL

Drug metabolism and disposition: the biological fate of chemicals 20220130 4

The proteomes of ordered and disordered lipid microdomains in rat liver microsomes from control and phenobarbital (PB)-treated rats were determined after solubilization with Brij 98 and analyzed by tandem mass tag (TMT)-liquid chromatography-mass spectrometry (LC-MS). This allowed characterization of the liver microsomal proteome and the effects of phenobarbital-mediated induction, focusing on quantification of the relative levels of the drug-metabolizing enzymes._The microsomal proteome from co ...[more]

PMID: 35094979

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