Proteomic study identified several proteins involved in cellular processes in recurrent otitis media

ABSTRACT: Recurrent otitis (ROM) is a pathological conditions in which a children have at least three episodes of acute otitis media over a course of 6 months. In this study we focused in proteins dysregulated between ROM and adenotonsillar hypertrophy (ATH). Tissue sample of seven ROM and seven ATH were obtained and submitted to two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) for protein identification and western-blotting for data validation. We identified 23 significantly (p<0.05) protein spots. The expression of two proteins were validated by western-blotting. The 2-DE coupled with MS approach identified several proteins involved in cellular and metabolic processes. Further study are needed to better understanding the role of this proteins in ROM.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Adenoid

DISEASE(S): Otitis Media

SUBMITTER: Giorgio Arrigoni  

LAB HEAD: Giorgio Arrigoni

PROVIDER: PXD022477 | Pride | 2020-12-10

REPOSITORIES: Pride