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Mass spectrometry analysis of two RNA-binding proteins, RBP23 and DRBD2, partners of different Poly(A) Binding proteins (PABP) homologues in Leishmania infantum

ABSTRACT: RNA binding proteins (RBP) have multiple roles associated with mRNA stability and translation. In the Leishmania genus, three Poly(A) Binding Proteins (PABP) were encoded with yet undefined functional distinctions but previous studies of mass-spectrometry identified RBPs differentially associated with PABP1 and PABP2, including RBP23 and DRBD2, respectively. Further characterization of both RBPs were performed through co-immunoprecipitation of proteins with HA-tagged RBP23 and DRBD2 from Leishmania infantum cytoplasmic extract cells, combined to mass spectrometry analysis. The analysis confirmed a strong association between RBP23 and PABP1, as well as with the EIF4E4/EIF4G3, a typical translation initiation complex, while DRBD2 was found to be more associated with PABP2 and a large number of different protein partners. Our studies expand the knowledge about RBPs function and their partners during Leishmania mRNA translation/metabolism.

INSTRUMENT(S): LTQ Orbitrap XL ETD

ORGANISM(S): Leishmania Infantum Jpcm5

SUBMITTER: Ludmila Arruda de Assis

LAB HEAD: Osvaldo Pompílio de Melo Neto

PROVIDER: PXD022480 | Pride | 2021-11-02

REPOSITORIES: Pride

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