High throughput and automated platform for MHC peptides purification
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Fusion Lumos, LTQ Orbitrap Elite
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): B Cell, Cell Culture
SUBMITTER: 
Lichao Zhang
LAB HEAD: Josh Elias
PROVIDER: PXD022629 | Pride | 2025-05-06
REPOSITORIES: Pride
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| FL0018659_manual_MHC1_HCD.raw | Raw | |||
| FL0018725_manual_MHC2_HCD.raw | Raw | |||
| FL0027174_raji_MHC1_50k_100ms.raw | Raw | |||
| FL0027176_raji_MHC1_50k_100ms.raw | Raw | |||
| FL0027178_raji_MHC1_30k_100ms.raw | Raw |
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Publications
Automated Ligand Purification Platform Accelerates Immunopeptidome Analysis by Mass Spectrometry.
Zhang Lichao L McAlpine Patrick L PL Heberling Marlene L ML Elias Joshua E JE
Journal of proteome research 20201217 1
Major histocompatibility complex (MHC)-presented peptides (pMHC) give insight into T cell immune responses, a critical step toward developing a new generation of targeted immunotherapies. Recent instrumentation advances have propelled mass spectrometry to being arguably the most robust technology for discovering and quantifying naturally presented pMHC from cells and tissues. However, sample preparation has remained a major limitation due to time-consuming and labor-intensive workflows. We devel ...[more]
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