Proteomics

Dataset Information

0

ULK1 on-bead in vitro kinase assay

ABSTRACT: Label-free phosphoproteomics of in vitro kinase assays. Three sets of bottom-up proteomics experiments were performed.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Joern Dengjel  

LAB HEAD: Joern Dengjel

PROVIDER: PXD022759 | Pride | 2021-11-03

REPOSITORIES: Pride

Json Xml
altmetric image

Publications

The evolutionarily conserved ULK1 kinase complex acts as gatekeeper of canonical autophagy and regulates induction of autophagosome biogenesis. To better understand control of ULK1 and analyze whether ULK1 has broader functions that are also linked to the later steps of autophagy, we perform comprehensive phosphoproteomic analyses. Combining in vivo with in vitro data, we identify numerous direct ULK1 target sites within autophagy-relevant proteins that are critical for autophagosome maturation  ...[more]

Similar Datasets

Proteomics In vitro kinase assay Pim1/GBP1
2023-09-26 | PXD030010 | Pride
Unknown Tethered-bead, immune sandwich assay.
| S-EPMC4150821 | biostudies-other
Proteomics Filamin C domain 18-21 in vitro kinase assay
2020-05-25 | PXD008893 | Pride
Proteomics Identification of phosphorylation sites of purified ORP8 from in vitro AMPK kinase assay
2023-10-24 | PXD036201 | Pride
Proteomics ULK1 regulated phosphatases in mouse and human cells
2021-11-03 | PXD022383 | Pride
Proteomics In vitro kinase assay to detect STX17 phosphorylation by ULK1
2023-06-27 | PXD039362 | iProX
Proteomics ALPK3 Whole Cell Lysate In vitro Kinase assay and phosphoproteomics
2023-01-19 | PXD038544 | Pride
Proteomics Large-scale in vitro kinase assay with FLNc d23-24 and PKC alpha
2017-01-02 | PXD003185 | Pride
Transcriptomics Separated Pancreatic stellate cells (PSCs) by functional separation using modified Matrigel invasion assay
2014-02-25 | GSE55300 | GEO
Unknown In situ single cell detection via microfluidic magnetic bead assay.
| S-EPMC5319813 | biostudies-literature