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Lysine acylations of humangut microbiome

ABSTRACT: In this study we developed an analytical workflow by integrating a serial immunoaffinity enrichment with high resolution mass spectrometry and unrestricted metaproteomic database search strategy, which enables efficient and comprehensive identification and quantification of lysine acetylation, propinoylation and succinylation in microbiomes.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Gut Metagenome

SUBMITTER: Xu Zhang

LAB HEAD: Daniel Figeys

PROVIDER: PXD022875 | Pride | 2021-08-26

REPOSITORIES: Pride

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Exploring the Microbiome-Wide Lysine Acetylation, Succinylation, and Propionylation in Human Gut Microbiota.

Zhang Xu X Cheng Kai K Ning Zhibin Z Mayne Janice J Walker Krystal K Chi Hao H Farnsworth Charles L CL Lee Kimberly K Figeys Daniel D

Analytical chemistry 20210422 17

Lysine acylations are important post-translational modifications that are present in both eukaryotes and prokaryotes and regulate diverse cellular functions. Our knowledge of the microbiome lysine acylation remains limited due to the lack of efficient analytical and bioinformatics methods for complex microbial communities. Here, we show that the serial enrichment using motif antibodies successfully captures peptides containing lysine acetylation, propionylation, and succinylation from human gut ...[more]

PMID: 33885279

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