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Target search for self-reactive antibodies in human renal allograft rejection

ABSTRACT: Self-reactive antibodies cloned from B cells infiltrating in a human renal allograft were subjected to IP-mass spectrometry using nuclear lysates of HEp-2 cells.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Donald Wolfgeher

LAB HEAD: Dr. Marcus R. Clark

PROVIDER: PXD022989 | Pride | 2021-04-27

REPOSITORIES: Pride

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Innate-like self-reactive B cells infiltrate human renal allografts during transplant rejection.

Asano Yuta Y Daccache Joe J Jain Dharmendra D Ko Kichul K Kinloch Andrew A Veselits Margaret M Wolfgeher Donald D Chang Anthony A Josephson Michelle M Cunningham Patrick P Tambur Anat A Khan Aly A AA Pillai Shiv S Chong Anita S AS Clark Marcus R MR

Nature communications 20210716 1

Intrarenal B cells in human renal allografts indicate transplant recipients with a poor prognosis, but how these cells contribute to rejection is unclear. Here we show using single-cell RNA sequencing that intrarenal class-switched B cells have an innate cell transcriptional state resembling mouse peritoneal B1 or B-innate (Bin) cells. Antibodies generated by Bin cells do not bind donor-specific antigens nor are they enriched for reactivity to ubiquitously expressed self-antigens. Rather, Bin ce ...[more]

PMID: 34272370

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