Name: Ross Sea Surface Water Metaproteomics
Keywords: Phytoplankton

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Ross Sea Surface Water Metaproteomics

ABSTRACT: We sampled the microbial community at the sea ice edge in McMurdo Sound, Ross Sea at the same location (-77.62S, 165.41E) for four weeks (as described in Wu et al 2019, Nat. Comms.). We had four sampling dates corresponding to weeks 1 to 4: December 28 2014, January 6, 15, and 22 2015. Large volumes of water (150--250 L) were filtered from 1 m depth at the sea ice edge, and passed through three filters sequentially (3.0, 0.8, and 0.1 um, each 293 mm Supor filters). Filters with collected biomass were then placed in tubes with a sucrose-based preservative buffer (20 mM EDTA, 400 mM NaCl, 0.75 M sucrose, 50 mM Tris-HCl, pH 8.0) and stored at -80 C until sample processing. We extracted proteins after buffer exchange into a 3\% SDS solution as previously described Wu et al 2019, Nat. Comms.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Marine Plankton Environmental Sample

SUBMITTER: Scott McCain

LAB HEAD: Erin M. Bertrand

PROVIDER: PXD022995 | Pride | 2021-09-07

REPOSITORIES: Pride

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Cellular costs underpin micronutrient limitation in phytoplankton.

McCain J Scott P JSP Tagliabue Alessandro A Susko Edward E Achterberg Eric P EP Allen Andrew E AE Bertrand Erin M EM

Science advances 20210806 32

Micronutrients control phytoplankton growth in the ocean, influencing carbon export and fisheries. It is currently unclear how micronutrient scarcity affects cellular processes and how interdependence across micronutrients arises. We show that proximate causes of micronutrient growth limitation and interdependence are governed by cumulative cellular costs of acquiring and using micronutrients. Using a mechanistic proteomic allocation model of a polar diatom focused on iron and manganese, we demo ...[more]

PMID: 34362734

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