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Structural and functional analyses identify unique features of the Smc5/6 complex among SMC family of complexes

ABSTRACT: Cross-linking mass spectrometry data for integrative structural analyses of the Smc5/6 holo-complex from budding yeast. Electron microscopy, crosslinking mass spectrometry, and computational modeling reveal that while the complex shares an overall organization with other SMC complexes, it possesses several strikingly different features.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Alex Kentsis

LAB HEAD: Alex Kentsis

PROVIDER: PXD023164 | Pride | 2021-05-05

REPOSITORIES: Pride

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Integrative analysis reveals unique structural and functional features of the Smc5/6 complex.

Yu You Y Li Shibai S Ser Zheng Z Sanyal Tanmoy T Choi Koyi K Wan Bingbing B Kuang Huihui H Sali Andrej A Kentsis Alex A Patel Dinshaw J DJ Zhao Xiaolan X

Proceedings of the National Academy of Sciences of the United States of America 20210501 19

Structural maintenance of chromosomes (SMC) complexes are critical chromatin modulators. In eukaryotes, the cohesin and condensin SMC complexes organize chromatin, while the Smc5/6 complex directly regulates DNA replication and repair. The molecular basis for the distinct functions of Smc5/6 is poorly understood. Here, we report an integrative structural study of the budding yeast Smc5/6 holo-complex using electron microscopy, cross-linking mass spectrometry, and computational modeling. We show ...[more]

PMID: 33941673

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