Proteomics

Dataset Information

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Mass spectrometry spatial-omics on a single conductive slide


ABSTRACT: Mass spectrometry imaging (MSI) can analyze the spatial distribution of hundreds of different molecules directly from tis-sue sections usually placed on conductive glass slides to provide conductivity on the sample surface. Additional experiments are often required for molecular identification using consecutive sections on membrane slides compatible with laser capture microdissection (LMD). In this work, we demonstrate for the first time the use of a single conductive slide for both matrix assisted laser desorption ionization (MALDI)-MSI and direct proteomics. In this workflow, regions of interest can be directly ablated with LMD while preserving protein integrity. These results offer an alternative for MSI based multimodal spatial-omics.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Rattus Norvegicus (rat) Mus Musculus (mouse)

TISSUE(S): Heart, Cardiac Muscle Cell

SUBMITTER: Stephanie Mezger  

LAB HEAD: Ronald Martinus Alexander Heeren

PROVIDER: PXD023196 | Pride | 2021-09-09

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
200220_Steph_LMD4_ITO_01.raw Raw
200220_Steph_LMD4_ITO_02.raw Raw
200220_Steph_LMD4_ITO_05.raw Raw
200220_Steph_LMD4_ITO_10.raw Raw
200220_Steph_LMD4_ITO_HE_01.raw Raw
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Publications

Mass Spectrometry Spatial-Omics on a Single Conductive Slide.

Mezger Stephanie T P STP   Mingels Alma M A AMA   Bekers Otto O   Heeren Ron M A RMA   Cillero-Pastor Berta B  

Analytical chemistry 20210107 4


Mass spectrometry imaging (MSI) can analyze the spatial distribution of hundreds of different molecules directly from tissue sections usually placed on conductive glass slides to provide conductivity on the sample surface. Additional experiments are often required for molecular identification using consecutive sections on membrane slides compatible with laser capture microdissection (LMD). In this work, we demonstrate for the first time the use of a single conductive slide for both matrix-assist  ...[more]

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