Dataset Information

Proteomic dataset of eggs of high and low quality in pikeperch, Sander lucioperca.

ABSTRACT: Gamete quality is one of the most important element of successful aquaculture. This especially applies to a newly domesticated fish species, such as pikeperch (Sander lucioperca) in which lowered and/or variable egg quality is one of the biggest obstacles toward rapid production expansion. However, mechanisms underlying pikeperch egg developmental competences remain unknown. Therefore, a quantitative proteome study was performed with high (HQ) and low (LQ) quality domesticated pikeperch eggs using a label free LC-MS/MS approach.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Sander Lucioperca

TISSUE(S): Egg

SUBMITTER: Thomas Fröhlich

LAB HEAD: Daniel Żarski

PROVIDER: PXD023229 | Pride | 2021-11-03

REPOSITORIES: Pride

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Publications

Neurodevelopment vs. the immune system: Complementary contributions of maternally-inherited gene transcripts and proteins to successful embryonic development in fish.

Żarski Daniel D Le Cam Aurelie A Frohlich Thomas T Kösters Miwako M Klopp Christophe C Nynca Joanna J Ciesielski Sławomir S Sarosiek Beata B Dryl Katarzyna K Montfort Jerome J Król Jarosław J Fontaine Pascal P Ciereszko Andrzej A Bobe Julien J

Genomics 20210909 6

The aim of this study was to investigate the respective contribution of maternally-inherited mRNAs and proteins to egg molecular cargo and to its developmental competence in fish using pikeperch as a model. Our study provides novel insights into the understanding of type-specific roles of maternally-inherited molecules in fish. Here we show, for the first time, that transcripts and proteins have distinct, yet complementary, functions in the egg of teleost fish. Maternally-inherited mRNAs would s ...[more]

PMID: 34508856

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Project description:Molecular profiling of the eggs is an excellent approach aiming at understanding biological processes and mechanisms conditioning developmental competence in finfishes and, consequently, reproductive fitness. Despite many efforts, it is still unclear what is the specific role of transcriptome and proteome in determination of egg quality in Teleostei fishes. The aim of this study was to perform integrated transcriptomic-proteomic analysis of eggs of pikeperch – a commercially relevant freshwater fish species. Additionally, comparative analysis of transcriptome and proteome in eggs characterized by either high or low egg quality has been performed, in order to identify mechanisms leading to early embryonic lethality in pikeperch. Our study provides a novel insight into the understanding of the role of maternally-derived molecular cargo in finfishes. The data obtained sheds light on the importance of transcriptome in development of nervous system suggesting neurogenesis-related mRNAs as a very important, non-genetic inheritance factor. Proteomic analysis highlights specific role of proteins in the immune response in ovulated eggs. Integrated analysis brings attention to the galactose-specific lectin nattectin gene and protein as the frontline defense molecule for the egg and developing embryo. The molecular analysis of egg developmental competence emphasizes post-vitellogenic processes (final oocyte maturation and ovulation) as the ones potentially compromising transcriptomic profile, but not affecting proteomic cargo. It highlights the need for careful reconsideration of the commercial reproductive protocols as the very strong modulators of egg quality and their molecular profile. Considering the mechanisms driving these alterations as well as consequences stemming from the differential abundance of the transcripts are still to be explored, the candidate quality markers, first time provided for pikeperch along with the current study, are creating valuable resource for further studies.

Project description:The domestication is an evolutionary process during which individuals will progressively adapt to an environment controlled by humans. It is accompanied by epigenetic and genetic modifications potentially leading to modifications in the transcriptomic profile in various tissues. The reproduction process is among the biological functions highly affected whatever the species and/or the way of domestication. Indeed, the reproduction success often decreases in domesticated populations. For fish, the molecular content of the eggs is crucial for the proper embryogenesis. Among these molecules the mRNA content depends directly from the gene expression and thus may reflect the genetic modifications occurring during this process. Here, we investigated the transcriptomic profile of eggs coming from females of two populations (F1 and Fx) that were reared in the same conditions and were genetically close. Our main goal was thus to determine how the domestication process may affect the reproduction success in the Eurasian perch and if it was mediated by the regulation of the transcription in the eggs. A regular follow up of the oogenesis progression showed few differences of the hormonal production between the two populations. Nevertheless, the histological study showed that the F1 females were more developed by the end of the oogenesis induction than the domesticated ones and as a result, they spawned earlier than the domesticated ones. The study of the early developmental success revealed significant differences between the populations. The microarray analysis of unfertilized eggs presented more than 350 transcripts differently expressed between the two populations. Among them, some specific genes were drastically more expressed in the domesticated (mex3B, hace1 and an uncharacterized protein) or F1 (per) populations. Our data show that the F1 population is more sensitive to the environmental conditions to advance oogenesis and reach the spawning season earlier. The transcriptomic profiles in eggs coming from the two populations were different and may lead to developmental impairments having a potential link with the domestication process. Further analyses are needed to explain these differences.

Dataset Information

Proteomic dataset of eggs of high and low quality in pikeperch, Sander lucioperca.

Publications

Neurodevelopment vs. the immune system: Complementary contributions of maternally-inherited gene transcripts and proteins to successful embryonic development in fish.

Similar Datasets

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