Proteomics

Dataset Information

0

Proteomic identification of recombinant nucleocapsid N protein 40 kDa fragment from SARS-CoV-2


ABSTRACT: Serological testing is important methodfor diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Nucleocapsid (N) protein is the most abundant virus derived protein and strong immunogen. We aimed to find its efficient, low-cost production. SARS-CoV-2 recombinant fragment of nucleocapsid protein (rfNP; 58-419 aa) was expressed in E. coli in soluble form, purified and characterized biochemically and immunologically. Purified rfNP has secondary structure of full-length recombinant N protein, with high percentage of disordered structure (34.2 %) and of β-sheet (40.7 %). rfNP was tested in immunoblot using sera of COVID-19 convalescent patients. ELISA was optimized with sera of RT-PCR confirmed positive symptomatic patients and healthy individuals. IgG detection sensitivity was 96% (47/50) and specificity 97% (67/68), while IgM detection was slightly lower (94% and 96.5%, respectively). Cost-effective approach for soluble recombinant N protein fragment production was developed, with reliable IgG and IgM antibodies detection of SARS-CoV-2 infection.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): SARS coronavirus Sin3765V  

TISSUE(S): Tissue Not Applicable To Dataset

DISEASE(S): Covid-19

SUBMITTER: Katarina Smiljanic  

LAB HEAD: Tanja D. Ćirković Velicković

PROVIDER: PXD023341 | Pride | 2021-02-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Figure 3 gel.tif Other
OB6993.mgf Mgf
OB6993.raw Raw
checksum.txt Txt
peptides_1_1_0.mzid.gz Mzid
Items per page:
1 - 5 of 5
altmetric image

Publications

Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2.

Djukic Teodora T   Mladenovic Maja M   Stanic-Vucinic Dragana D   Radosavljevic Jelena J   Smiljanic Katarina K   Sabljic Ljiljana L   Devic Marija M   Cujic Danica D   Vasovic Tamara T   Simovic Ana A   Radomirovic Mirjana M   Cirkovic Velickovic Tanja T  

Virology 20210209


Serological testing is important method for diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. Nucleocapsid (N) protein is the most abundant virus derived protein and strong immunogen. We aimed to find its efficient, low-cost production. SARS-CoV-2 recombinant fragment of nucleocapsid protein (rfNP; 58-419 aa) was expressed in E. coli in soluble form, purified and characterized biochemically and immunologically. Purified rfNP has secondary structure of full-leng  ...[more]

Similar Datasets

2021-01-01 | S-EPMC7871913 | BioStudies
2009-01-01 | S-EPMC2643541 | BioStudies
2020-01-01 | S-EPMC7367508 | BioStudies
2004-01-01 | S-EPMC7130062 | BioStudies
2020-01-01 | S-EPMC7445483 | BioStudies
2021-01-01 | S-EPMC7854735 | BioStudies
2020-01-01 | S-EPMC7184472 | BioStudies
2020-01-01 | S-EPMC7273175 | BioStudies
2020-01-01 | S-EPMC7600936 | BioStudies
2020-01-01 | S-EPMC7461757 | BioStudies