Comprehensive Analysis of Tryptic Peptides Arising from Disulfide Linkages in NISTmAb

ABSTRACT: This work presents methods for identifying and then creating a mass spectral library for disulfide-linked peptides originating from the NISTmAb, a reference material of humanized IgG1k monoclonal antibody (RM 8671). Analyses involved both partially-reduced and non-reduced samples under neutral and weakly basic conditions, followed by nanoflow liquid chromatography tandem mass spectrometry (LC-MS/MS).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Qian Dong  

LAB HEAD: Stephen E. Stein

PROVIDER: PXD023358 | Pride | 2021-02-08

REPOSITORIES: Pride