Proteomics

Dataset Information

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A long-term 3D organoid (tubuloid) model of the adult mouse kidney


ABSTRACT: The aim of the project was the analysis of the bulk proteome and phosphoproteome in 3D organoids (tubuloids) from the adult mouse kidney. Proteome (nearly 9000 proteins) and phosphoproteome (nearly 16000 phospho sites) were compared in three biological (independent) replicates of epithelial organoids (early passage and long-term, 3-month cultures) and freshly isolated, MAC-sorted, Epcam-positive kidney epithelial cells. After collection of the organoids, Matrigel was removed using a non-enzymatic solution.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Epithelial Cell, Kidney

SUBMITTER: Oliver Popp  

LAB HEAD: Walter Birchmeier

PROVIDER: PXD023491 | Pride | 2024-02-26

REPOSITORIES: Pride

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