HyperLOPIT and temporal proteomic profiling of the response to lipopolysaccharide in the THP-1 human leukaemia cell line
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): B Cell, Blood
SUBMITTER: 
Claire Mulvey
LAB HEAD: Kathryn Susan Lilley
PROVIDER: PXD023509 | Pride | 2022-02-16
REPOSITORIES: Pride
Publications
Spatiotemporal proteomic profiling of the pro-inflammatory response to lipopolysaccharide in the THP-1 human leukaemia cell line.
Mulvey Claire M CM Breckels Lisa M LM Crook Oliver M OM Sanders David J DJ Ribeiro Andre L R ALR Geladaki Aikaterini A Christoforou Andy A Britovšek Nina Kočevar NK Hurrell Tracey T Deery Michael J MJ Gatto Laurent L Smith Andrew M AM Lilley Kathryn S KS
Nature communications 20211001 1
Protein localisation and translocation between intracellular compartments underlie almost all physiological processes. The hyperLOPIT proteomics platform combines mass spectrometry with state-of-the-art machine learning to map the subcellular location of thousands of proteins simultaneously. We combine global proteome analysis with hyperLOPIT in a fully Bayesian framework to elucidate spatiotemporal proteomic changes during a lipopolysaccharide (LPS)-induced inflammatory response. We report a hi ...[more]
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