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RNA antisnse puriification of Tau exon 10 pre-mRNA

ABSTRACT: In this study, we demonstrated the use of the RNA Antisense Purification by Mass Spectrometry (RAP-MS) method to identify proteins involved in specific alternative splicing events. Specifically, we applied the RAP-MS method to identify proteins that interact with the Tau pre-mRNA around its exon 10 region.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Yu Lu

LAB HEAD: Yu Lu

PROVIDER: PXD023523 | Pride | 2022-02-16

REPOSITORIES: Pride

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Identification of HnRNPC as a novel Tau exon 10 splicing factor using RNA antisense purification mass spectrometry.

Xing Sansi S Wang Jane J Wu Ruilin R Hefti Marco M MM Crary John F JF Lu Yu Y

RNA biology 20211231 1

Alternative splicing in Tau exon 10 generates 3 R- and 4 R-Tau proteoforms, which have equal abundance in healthy adult human brain. Aberrant alternative splicing in Tau exon 10 leads to distortion of the balanced 3 R- and 4 R-Tau expression levels, which is a causal factor to trigger toxic Tau aggregation, neuron dysfunction and patient death in a group of neurodegenerative diseases known as tauopathies. Hence, identification of regulators upstream of the Tau exon 10 splicing events are crucial ...[more]

PMID: 34965173

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