Proteomics

Dataset Information

0

Defining Essential Regulators of Naïve Human Pluripotent Cell Reprogramming


ABSTRACT: Defining how human pluripotent cell identity is controlled and in particular how naïve pluripotency is acquired during cell reprogramming is crucial for the future applications of pluripotent stem cells. However, the regulatory pathways of naïve cell reprogramming remain incompletely understood. Here, we used genome-wide CRISPR-Cas9 screening to identify novel regulators of primed to naïve pluripotent stem cell reprogramming, including genes that are essential for reprogramming and genes that normally impede reprogramming and whose targeted deletion led to enhanced reprogramming. Integrated analysis defined specific chromatin complexes and signalling pathways as critical regulators of naïve reprogramming, and were largely distinct from regulators of somatic cell reprogramming. Mechanistically, PRC1.3 and PRDM14 are jointly required to transcriptionally repress developmental and gene regulatory factors to ensure naïve cell reprogramming. Additionally, small molecule inhibitors of reprogramming impediments increased the efficiency of naïve cell reprogramming, and are of practical benefit that can improve on current reprogramming methods. Taken together, we have identified novel regulators controlling the establishment of naïve pluripotency in human cells, which will open up new ways to exploit the full potential of pluripotent stem cells. These results also provide new insights into mechanisms that destabilise and reconfigure cell identity during cell state transitions.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Stem Cell

DISEASE(S): Disease Free

SUBMITTER: Valar Nila Roamio Franklin  

LAB HEAD: Clive D' Santos

PROVIDER: PXD023547 | Pride | 2022-04-06

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Lumos_HPSC_16Plex_Fraction_01.raw Raw
Lumos_HPSC_16Plex_Fraction_02.raw Raw
Lumos_HPSC_16Plex_Fraction_03.raw Raw
Lumos_HPSC_16Plex_Fraction_04.raw Raw
Lumos_HPSC_16Plex_Fraction_05.raw Raw
Items per page:
1 - 5 of 11

Similar Datasets

2015-12-31 | E-GEOD-67520 | biostudies-arrayexpress
2019-08-16 | E-MTAB-8046 | biostudies-arrayexpress
2015-04-15 | E-GEOD-67710 | biostudies-arrayexpress
2022-03-25 | GSE164786 | GEO
2015-09-01 | E-GEOD-68798 | biostudies-arrayexpress
2019-08-16 | E-MTAB-7901 | biostudies-arrayexpress
| PRJNA691952 | ENA
2015-12-10 | E-GEOD-72744 | biostudies-arrayexpress
2012-12-20 | E-GEOD-42477 | biostudies-arrayexpress
2015-04-15 | E-GEOD-67715 | biostudies-arrayexpress