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MicroRNA-mediated translation repression through GYF-1 and IFE-4 in C. elegans development

ABSTRACT: Proteomic mass spectrometry was used to identify interactors of the miRNRA-Induced Silencing Complex in C. elegans.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Whole Body

SUBMITTER: Yasaman Jami-Alahmadi

LAB HEAD: Thomas Duchaine

PROVIDER: PXD023610 | Pride | 2021-09-10

REPOSITORIES: Pride

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microRNA-mediated translation repression through GYF-1 and IFE-4 in C. elegans development.

Mayya Vinay K VK Flamand Mathieu N MN Lambert Alice M AM Jafarnejad Seyed Mehdi SM Wohlschlegel James A JA Sonenberg Nahum N Duchaine Thomas F TF

Nucleic acids research 20210501 9

microRNA (miRNA)-mediated gene silencing is enacted through the recruitment of effector proteins that direct translational repression or degradation of mRNA targets, but the relative importance of their activities for animal development remains unknown. Our concerted proteomic surveys identified the uncharacterized GYF-domain encoding protein GYF-1 and its direct interaction with IFE-4, the ortholog of the mammalian translation repressor 4EHP, as key miRNA effector proteins in Caenorhabditis ele ...[more]

PMID: 33758928

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