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Proteomics of biphenyl labelling study

ABSTRACT: protein based stable isotope probing was performed for identifying microorganisms actually responsible for biphenyl biodegradation in soil environment.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Soil Metagenome

SUBMITTER: Songcan Chen

LAB HEAD: Florin Musat

PROVIDER: PXD023646 | Pride | 2022-02-16

REPOSITORIES: Pride

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Novel clades of soil biphenyl degraders revealed by integrating isotope probing, multi-omics, and single-cell analyses.

Chen Song-Can SC Budhraja Rohit R Adrian Lorenz L Calabrese Federica F Stryhanyuk Hryhoriy H Musat Niculina N Richnow Hans-Hermann HH Duan Gui-Lan GL Zhu Yong-Guan YG Musat Florin F

The ISME journal 20210611 12

Most microorganisms in the biosphere remain uncultured and poorly characterized. Although the surge in genome sequences has enabled insights into the genetic and metabolic properties of uncultured microorganisms, their physiology and ecological roles cannot be determined without direct probing of their activities in natural habitats. Here we employed an experimental framework coupling genome reconstruction and activity assays to characterize the largely uncultured microorganisms responsible for ...[more]

PMID: 34117322

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