Identification of proteins precipitated by means of Acyl-RAC

ABSTRACT: The objective was to perform protein identification by ESI-LC-MSMS on a sample subjected to Acyl-RAC assay in order to study protein palmitoylation. The user transfected human paxillin (mCherry-paxillin-CAIL) into a mouse cell line and performed Acyl-RAC assay. The aim of this analysis was to determine whether human paxillin is palmitoylated and confirm the quality of the Acyl-RAC technique to detect palmitoylated proteins.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Fibroblast

SUBMITTER: Bernhard Wehrle-Haller  

LAB HEAD: Bernhard Wehrle-Haller

PROVIDER: PXD023810 | Pride | 2021-03-30

REPOSITORIES: Pride