Ontology highlight
ABSTRACT:
INSTRUMENT(S): timsTOF Pro
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Epithelial Cell, Cell Culture, Skin, Saliva
DISEASE(S): Cutaneous Malignant Melanoma 1
SUBMITTER: Fabian Coscia
LAB HEAD: Matthias Mann
PROVIDER: PXD023904 | Pride | 2022-04-27
REPOSITORIES: Pride
Mund Andreas A Coscia Fabian F Kriston András A Hollandi Réka R Kovács Ferenc F Brunner Andreas-David AD Migh Ede E Schweizer Lisa L Santos Alberto A Bzorek Michael M Naimy Soraya S Rahbek-Gjerdrum Lise Mette LM Dyring-Andersen Beatrice B Bulkescher Jutta J Lukas Claudia C Eckert Mark Adam MA Lengyel Ernst E Gnann Christian C Lundberg Emma E Horvath Peter P Mann Matthias M
Nature biotechnology 20220519 8
Despite the availabilty of imaging-based and mass-spectrometry-based methods for spatial proteomics, a key challenge remains connecting images with single-cell-resolution protein abundance measurements. Here, we introduce Deep Visual Proteomics (DVP), which combines artificial-intelligence-driven image analysis of cellular phenotypes with automated single-cell or single-nucleus laser microdissection and ultra-high-sensitivity mass spectrometry. DVP links protein abundance to complex cellular or ...[more]