Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Exploring the glycosylation state of the Cryptosporidium parvum CpTSP protein family-Cman pulldowns

ABSTRACT: Cryptosporidium parvum is a zoonotic apicomplexan parasite and a common cause of diarrheal disease worldwide. The development of vaccines to prevent or limit infection remains an important goal for tackling these diarrheal diseases, which are a significant cause of infant morbidity in the developing world. The only approved vaccine against an apicomplexan parasite targets conserved adhesins possessing a thrombospondin repeat (TSR) domains. Orthologous TSR domain-containing proteins are commonplace in the apicomplexa and C. parvum possess 12 such proteins. Here, we explore the molecular evolution and conservation of these proteins and examine their abundance in C. parvum oocysts to assess the likelihood that they may be useful as vaccine candidates. We go onto examine the glycosylation states of these proteins using antibody-enabled and ZIC-HILIC enrichment techniques, which revealed that these proteins are modified with C-linked Hex and N-linked Hex5-6HexNAc2 glycans.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Cryptosporidium Parvum Iowa

SUBMITTER: Nichollas Scott

LAB HEAD: Nichollas Scott

PROVIDER: PXD023934 | Pride | 2023-01-12

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Similar Datasets

Exploring the glycosylation state of the Cryptosporidium parvum CpTSP protein family-FAIMS proteome

Project description:Cryptosporidium parvum is a zoonotic apicomplexan parasite and a common cause of diarrheal disease worldwide. The development of vaccines to prevent or limit infection remains an important goal for tackling these diarrheal diseases, which are a significant cause of infant morbidity in the developing world. The only approved vaccine against an apicomplexan parasite targets conserved adhesins possessing a thrombospondin repeat (TSR) domains. Orthologous TSR domain-containing proteins are commonplace in the apicomplexa and C. parvum possess 12 such proteins. Here, we explore the molecular evolution and conservation of these proteins and examine their abundance in C. parvum oocysts to assess the likelihood that they may be useful as vaccine candidates. We go onto examine the glycosylation states of these proteins using antibody-enabled and ZIC-HILIC enrichment techniques, which revealed that these proteins are modified with C-linked Hex and N-linked Hex5-6HexNAc2 glycans.

2023-01-12 | PXD023935 | Pride

Exploring the glycosylation state of the Cryptosporidium parvum CpTSP protein family-HILIC glycopeptide enrichment

2023-01-12 | PXD023936 | Pride

Discovery of a Novel Human Polyomavirus in Acute Diarrheal Samples from Children

Project description:By unbiased deep sequencing, we identified a novel, highly divergent polyomavirus, provisionally named MX polyomavirus (MXPyV), in stool samples from children. From Mexico, 12 samples (out of 96) were positive for MxPyV by MXPyV-specific PCR. We used the ViroChip microarray and PCR to screen these 12 samples for co-infection with common diarrheal viruses. Six of 12 MxPyV-positive diarrheal samples tested negative by the ViroChip and PCR, and the other 6 samples were positive for at least one known diarrheal virus. The ViroChip microarray (version 5.0, Viro5AG-60K platform, GPL15905) was used to screen RNA extracts from MX polyomavirus (MXPyV)-positive pediatric diarrheal samples from Mexico for common diarrheal viruses.

2012-10-01 | E-GEOD-40008 | biostudies-arrayexpress

Glucocorticoid receptor profiling (ChIP-seq) in U2OS-GR cells 24 hours after hormone washout

Project description:We performed ChIP-seq targeting the glucocorticoid receptor (GR) in the U2OS-GR cell line. The cell line is derived from U2OS ATTC:HTB-96 and stably transfected with an expression construct for rat GR. The cells were treated with 100 nM dexamethasone for 4 hours, washed 2x with PBS and cultured in hormone-free medium for 24 hours before harvest.

2021-05-03 | E-MTAB-9914 | biostudies-arrayexpress

Glucocorticoid and androgen receptor profiling (ChIP-seq) in U2OS-GR and U2OS-AR cells upon hormone treatment

Project description:We performed ChIP-seq targeting the glucocorticoid receptor (GR) in the U2OS-GR cell line and the androgen receptor (AR) in the U2OS-AR cell line. The cell lines are derived from U2OS ATTC:HTB-96 and stably transfected with an expression construct for either rat GR or human AR, respectively. The U2OS-GR cells were treated with dexamethasone (1 µM) for 90 minutes. The U2OS-AR cells were treated with R1881 (5 nM) for 4 hours.

2021-03-09 | E-MTAB-9616 | biostudies-arrayexpress

Mouse expression data of epididymal white adipose tissue at Zeitgeber time (ZT) 18 comparison control vs. Sleep restriction

Project description:We hybridzed cRNA from epididymal white adipose tissue collected at ZT18 of control animals and TSR animals (TSR: these mice were sleep restricted for 6 hours every day by gentle handling for 5 consecutive days and killed on the last day at ZT18) mice used in this study were C57BL/6 control mice were compared to timed sleep restriction mice (TSR: these mice were sleep restricted for 6 hours every day by gentle handling for 5 consecutive days and killed on the last day at ZT18)

2015-06-15 | E-GEOD-38921 | biostudies-arrayexpress

Mouse expression data of epididymal white adipose tissue at Zeitgeber time (ZT) 18 comparison control vs. Sleep restriction

2015-06-15 | GSE38921 | GEO

Identification of glutamate receptor delta-1 interactome at synapses.

Project description:In this project, we have investigated the interactome of the glutamate receptor delta-1 (GluD1) in developing synapses in mice. GluD1 is a member of the delta subfamily of ionotropic glutamate receptors widely expressed in the brain. It behaves as a postsynaptic organizer by engaging in trans-synaptic interaction and by mediating postsynaptic signaling.

2019-11-06 | PXD010373 | Pride

A genetic screen identifies a protective type III interferon response to Cryptosporidium that requires TLR3 dependent recognition

Project description:Cryptosporidium is a leading cause of severe diarrhea and diarrheal-related death in children worldwide. As an obligate intracellular parasite, Cryptosporidium relies on intestinal epithelial cells to provide a niche for its growth and survival, but little is known about the contributions that the infected cell makes to this relationship. Here we conducted a genome wide CRISPR/Cas9 knockout screen to discover host genes required for Cryptosporidium parvum infection and/or host cell survival. The gene enrichment analysis indicated that the host interferon response, glycosaminoglycan (GAG) and glycosylphosphatidylinositol (GPI) anchor biosynthesis are important determinants of susceptibility to C. parvum infection. Several of these pathways are linked to parasite attachment and invasion and C-type lectins on the surface of the parasite. Evaluation of transcript and protein induction of innate interferons revealed a pronounced type III interferon response to Cryptosporidium in human cells as well as in mice. Treatment of mice with IFNλ reduced infection burden and protected immunocompromised mice from severe outcomes including death, with effects that STAT1 signaling in the enterocyte. Initiation of this type III interferon response was dependent on sustained intracellular growth and mediated by the pattern recognition receptor TLR3. We conclude that host cell intrinsic recognition of Cryptosporidium results in IFNλ production critical to early protection against this infection.

2021-10-06 | GSE185247 | GEO

Oligoarray analysis of mRNA species from DCs treated with C. parvum for 24 h.

Project description:To investigate whether C. parvum treatment of DCs regulates other inflammatory genes, mouse BMDCs (CD11c and CD11b positive) were treated with C. parvum for 24 hr. Control groups included untreated BMDCs and DCs treated with E. cuniculi. We noticed that a significant upregulation of the expression of interferon-related genes, which may correlation the critical role of interferon in host defense against C. parvum infection. DCs were treated with C. parvum for 24 hr. Control groups included untreated BMDCs and DCs treated with E. cuniculi. Group comparison was performed using the data of 3 replicate arrays from each time point.

2010-10-20 | E-GEOD-24121 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data