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The phosphorylation of the Smad2/3 linker region by Nemo-like kinase regulates TGF-β signaling

ABSTRACT: Smad2 and Smad3 (Smad2/3) primarily mediates the transforming growth factor-β (TGF-β) signaling that drives cell proliferation, differentiation, and migration. The dynamics of the Smad2/3 phosphorylation provides the key mechanism for regulating the TGF-β signaling pathway. Here we identified NLK as a novel regulator of TGF-β signaling pathway via modulating the phosphorylation of Smad2/3 in the linker region.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Yanchi Zhou

LAB HEAD: Wei Song

PROVIDER: PXD023957 | Pride | 2021-09-10

REPOSITORIES: Pride

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The phosphorylation of the Smad2/3 linker region by nemo-like kinase regulates TGF-β signaling.

Liang Junbo J Zhou Yanchi Y Zhang Ning N Wang Dingding D Cheng Xiaowen X Li Kai K Huang Rong R Lu Yan Y Wang Hailong H Han Deqiang D Wu Wei W Han Meng M Miao Shiying S Wang Linfang L Zhao Hong H Song Wei W

The Journal of biological chemistry 20210101

Smad2 and Smad3 (Smad2/3) are structurally similar proteins that primarily mediate the transforming growth factor-β (TGF-β) signaling responsible for driving cell proliferation, differentiation, and migration. The dynamics of the Smad2/3 phosphorylation provide the key mechanism for regulating the TGF-β signaling pathway, but the details surrounding this phosphorylation remain unclear. Here, using in vitro kinase assay coupled with mass spectrometry, we identified for the first time that nemo-li ...[more]

PMID: 33676893

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