Proteomics

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Bursaphelenchus xylophilus secretome under Pinus pinaster and P. pinea stimuli


ABSTRACT: The pinewood nematode, Bursaphelenchus xylophilus, the pine wilt disease's causal agent, is a migratory endoparasitic nematode skilled to feed on pine tissues and on fungi that colonize the trees. In order to study B. xylophilus secretomes under the stimulus of pine species with different susceptibility to disease, nematodes were exposed to aqueous pine extracts from Pinus pinaster (high susceptible host) and P. pinea (low susceptible host). Sequential windowed acquisition of all theoretical mass spectra (SWATH-MS) was used to determine relative changes in protein amounts between B. xylophilus secretions, and a total of 776 secreted proteins were quantified in both secretomes.

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Bursaphelenchus Xylophilus (pinewood Nematode Worm) (aphelenchoides Xylophilus)

TISSUE(S): Secretion

SUBMITTER: Sandra Anjo  

LAB HEAD: Joana Sá

PROVIDER: PXD024011 | Pride | 2021-04-27

REPOSITORIES: Pride

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Publications

Comparative Analysis of <i>Bursaphelenchus xylophilus</i> Secretome Under <i>Pinus pinaster</i> and <i>P. pinea</i> Stimuli.

Silva Hugo H   Anjo Sandra I SI   Manadas Bruno B   Abrantes Isabel I   Fonseca Luís L   Cardoso Joana M S JMS  

Frontiers in plant science 20210511


The pinewood nematode (PWN), <i>Bursaphelenchus xylophilus</i>, the pine wilt disease's (PWD) causal agent, is a migratory endoparasitic nematode skilled to feed on pine tissues and on fungi that colonize the trees. In order to study <i>B. xylophilus</i> secretomes under the stimulus of pine species with different susceptibilities to disease, nematodes were exposed to aqueous pine extracts from <i>Pinus pinaster</i> (high-susceptible host) and <i>P. pinea</i> (low-susceptible host). Sequential w  ...[more]

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