Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomics in mutant KRAS Lung cancer

ABSTRACT: Proteomic studies to check the effect of MEK1/2 and KRAS G12C inhibitors in lung cancer cells

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Lung, Cell Culture

DISEASE(S): Lung Cancer

SUBMITTER: Enrique SantamarÃa

LAB HEAD: Enrique Santamaria

PROVIDER: PXD024023 | Pride | 2023-08-22

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Deamidation__NQ_Sites.txt	Txt
	F21-178-_NMPs_TMT_Plex_4-1.wiff	Wiff
	F21-178-_NMPs_TMT_Plex_4-1.wiff.scan	Wiff
	F22-178-_NMPs_TMT_Plex_1-1.wiff	Wiff
	F22-178-_NMPs_TMT_Plex_1-1.wiff.scan	Wiff

Items per page:

1 - 5 of 251

Similar Datasets

Expression Analysis of Trimethyltin Chlorides (TMT) Impact on Embryonic Development

Project description:Investigation of whole genome expression pattern of 60 and 72 hours post fertilization Danio Rerio embryos exposed to TMT and vehicle control Embryos were exposed to 10uM TMT or control from 48hpf to 60 or 72 hpf. Three replicates were collected for each time point. 40 embryos were pooled to comprise a replicate.

2011-03-23 | E-GEOD-28131 | biostudies-arrayexpress

Transcription profiling of mouse wild type adult male mouse livers vs transgenic mice expressing human Small Heterodimer Partner in hepatocytes

Project description:Comparison of gene expression in adult male mouse livers between wildtype mice and Transgenic mice expressing human Small Heterodimer Partner in hepatocytes.

2006-01-01 | E-MEXP-294 | biostudies-arrayexpress

Thermal Proteome Profiling Datasets for smarTPCA

Project description:Chemical biology and the application of small molecules has proven to be a potent perturbation strategy especially for the functional elucidation of proteins, their networks and regulators. In recent years, the cellular thermal shift assay (CETSA) and its proteome-wide extension, thermal proteome profiling (TPP), have proven to be effective tools for identifying interactions of small molecules with their target proteins as well as off-targets in living cells. Here, we asked the question if isothermal dose-response (ITDR) CETSA can be exploited to characterize secondary effects downstream of the primary binding event, such as changes in post-translational modifications or protein-protein interactions (PPI). Applying ITDR-CETSA to MAPK14 kinase inhibitor treatment of living HL-60 cells, we found similar dose-responses for the direct inhibitor target and its known interaction partners MAPKAPK2 and MAPKAPK3. Extension of the dose-response similarity comparison to the proteome wide level using TPP with compound concentration range (TPP-CCR) revealed not only the known MAPK14 interaction partners MAPKAPK2 and MAPKAPK3, but also the potentially new intracellular interaction partner MYLK. We are confident that dose-dependent small molecule treatment in combination with ITDR-CETSA or TPP-CCR similarity assessment will not only allow discrimination between primary and secondary effects, but also provide a novel method to study PPI in living cells without perturbation by protein modification, which we named "small molecule arranged thermal proximity coaggregation" (smarTPCA).

2022-06-09 | PXD032942 | Pride

Whole-exome sequencing of genomic DNA extracted from patient-derived melanoma cell lines resistant to vemurafenib (PLX) or trametinib (TRA)

Project description:Whole-exome sequencing was performed on DNA samples extracted from seven melanoma cell lines resistant to either vemurafenib (BRAF V600E inhibitor) or trametinib (MEK1/2 inhibitor). The aim of the experiment was to search for genetic alterations responsible for phenotypic diversity of melanoma cell lines reported at the level of cell morphology, activity of signaling pathways essential for melanoma development and progression, and resistance to targeted therapeutics.

2018-10-20 | E-MTAB-7248 | biostudies-arrayexpress

Whole-exome sequencing of genomic DNA from patient-derived melanoma cell lines resistant to either vemurafenib (PLX) or trametinib (TRA).

2019-06-15 | E-MTAB-7991 | biostudies-arrayexpress

Whole-exome sequencing of genomic DNA extracted from patient-derived melanoma cell lines resistant to vemurafenib (PLX).

Project description:Whole-exome sequencing was performed on DNA sample extracted from one melanoma cell line resistant to vemurafenib (BRAF V600E inhibitor). The aim of the experiment was to search for genetic alterations responsible for phenotypic diversity of melanoma cell lines reported at the level of cell morphology, activity of signaling pathways essential for melanoma development and progression, and resistance to targeted therapeutics.

2019-07-25 | E-MTAB-8150 | biostudies-arrayexpress

Proteomics analysis in GGT subjects

Project description:Proteomic analysis of brain structures derived from GGT subjects

2020-05-26 | PXD015768 | Pride

Transcription profiling of rat at pheochromocytoma cell line PC12 treated with trimethyltin at low and high doses

Project description:The rat pheochromocytoma cell line PC12 cells were cultured in complete DMEM till 80% confluence, then placed at 5000 cells per squared cm. Cells were then plated in 24-well plates for cell viability assay and in T75 flasks for RNA isolation. Medium was replaced with serum-free fresh medium for 12 hours prior to TMT treatment. Gene expression patterns were then analysed using Rat Expression Array 230A Experiment Overall Design: In this study we analize gene expression patterns in PC12 cells treated with Trimethyltin (TMT). We utilized control cells (untreated) and two different concentration (1 and 5) Experiment Overall Design: We used three biological replicates, for the three concentration tested, according to MIAME guidelines Experiment Overall Design: (total 9 chips were used in this study).

2009-04-29 | E-GEOD-5073 | biostudies-arrayexpress

Transcription profiling of Mycobacterium tuberculosis H37Rv and the isogenic mprA::Kmr mutant

Project description:Whole genome expression analyses between wild-type Mycobacterium tuberculosis H37Rv and the isogenic mprA::Kmr mutant. Expression analyses between these strains were conducted following growth under physiological conditions, or following exposure to non-inhibitory concentrations of SDS or Triton X-100.

2006-03-01 | E-MEXP-552 | biostudies-arrayexpress

Transcription profiling of dissected neuroepithelium (Neur.) and from all other non-neuroepithelial tissues (N. Neur for Non Neuronal tissues) from six wild type (WT) and six Mdm4-null mouse embryos (KO)

Project description:Total RNA was isolated from the dissected neuroepithelium (Neur.) and from all other non-neuroepithelial tissues (N. Neur for Non Neuronal tissues) from six wild-type (WT) and six Mdm4-null embryos (KO). Equal quantities of total RNA from single samples were used to obtain four RNA pools (WT-Neur, WT-N.Neur, KO-Neur, KO-N.Neur), each representing one of the experimental samples under analysis. Biotin labeled cRNA targets were obtained starting from 10ug of total RNA derived from the RNA pools. cDNA synthesis was performed with GIBCO SuperScript Custom cDNA Synthesis Kit, and biotin-labeled antisense RNA was transcribed in vitro using Ambion's In Vitro Transcription System, and including Bio-16-UTP and Bio-11-CTP (Enzo) in the reaction. Each labeled target was hybridized to two copies of the the complete Affymetrix MG-U174v2 chip set.

2004-12-31 | E-MEXP-155 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data