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Effect of ATP2 deletion on protein expression in Candida albicans

ABSTRACT: To investigate the effect of ATP2 knockdown on the protein expression of Candida albicans, we performed proteomic assays for atp2Δ/Δ and wild type strains. A total of 4000.0 proteins were identified in this study, of which 3652.0 proteins contained quantitative information. If a 1.5-fold differential expression change threshold and a statistical test t-test p-value < 0.05 were used as the significance threshold, then among the quantified proteins, we found that 112 proteins were up-regulated and 268 proteins were down-regulated in atp2Δ/Δ cells. Based on the above data, we performed systematic bioinformatics analysis (protein functional annotation) of all identified proteins, and performed functional classification, functional enrichment and functional enrichment-based clustering analysis of all differentially expressed proteins.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Candida Albicans (yeast)

SUBMITTER: Yishan Zhang

LAB HEAD: Hong Zhang

PROVIDER: PXD024039 | Pride | 2021-09-10

REPOSITORIES: Pride

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Deletion of the ATP2 Gene in Candida albicans Blocks Its Escape From Macrophage Clearance.

Zhang Yishan Y Tang Chuanyan C Zhang Zhanpeng Z Li Shuixiu S Zhao Yajing Y Weng Luobei L Zhang Hong H

Frontiers in cellular and infection microbiology 20210416

Macrophages provide the first-line defense against invasive fungal infections and, therefore, escape from macrophage becomes the basis for the establishment of Candida albicans invasive infection. Here, we found that deletion of ATP2 (atp2Δ/Δ) in C. albicans resulted in a dramatic decrease from 69.2% (WT) to 1.2% in the escape rate in vitro. The effect of ATP2 on macrophage clearance stands out among the genes currently known to affect clearance. In the ...[more]

PMID: 33937095

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