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Escherichia coli proteome upon sudden carbon starvation

ABSTRACT: We studied the proteome changes in Escherichia coli growing in rich media, and compared the changes between exponential phase cells and those underwent sudden carbon starvations.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Escherichia Coli

SUBMITTER: Handuo Shi

LAB HEAD: Kerwyn Casey Huang

PROVIDER: PXD024151 | Pride | 2021-03-09

REPOSITORIES: Pride

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Escherichia coli proteome during outgrowth from stationary phase

Project description:We studied the proteomic dynamics of Escherichia coli cells exiting stationary phase and identified unique dynamics for the proteins.

2021-01-22 | PXD023729 | Pride

METTL21C methylates Alanine tRNA synthetase at lysine 943 in muscle tissue

Project description:Protein lysine methylation is a common post-translational modification (PTM) detected throughout the human proteome that plays important roles in diverse biological processes. In the human genome, there are greater than one hundred known and candidate protein lysine methyltransferases (PKMTs), many with important links to human disease. METTL21C (methyltransferase-like protein 21C) is a PKMT implicated in muscle biology and reported to methylate VCP (valosin-containing protein/p97) and HSPA8 (heat shock 70kDa protein 8). However, a clear in vitro methyltransferase activity for METTL21C is yet to be demonstrated. Thus, whether METTL21C is indeed an active enzyme that directly methylates substrate/s is unclear. Here, we use an unbiased biochemical-based screening assay coupled to mass spectrometry to identify Alanine-tRNA-Synthetase 1 (AARS1) as a direct substrate of METTL21C. METTL21C catalyzes mono-, di- and tri-methylation of lysine 943 of AARS1 (AARS1-K943me) in vitro and in vivo. In vitro methylation of AARS1 by METTL21C is independent of ATP or tRNA molecules. In contrast to AARS1, and in conflict with previous reports, we do not detect METTL21C methylation activity on VCP and HSPA8. Depletion of METTL21C in METTL21C-expressing cells leads to depletion of AARS1-K943 methylation. Finally, METTL2C is almost exclusively expressed in muscle tissue, and accordingly we detect the METTL21C-catalyzed methylation event of AARS1 in mouse skeletal muscle tissue. Together, our work identifies AARS1 as a bona fide substrate of METTL21C and suggests a role for the METTL21C-AARS1 axis in the regulation of protein synthesis in muscle tissue. In addition, our study describes a straightforward protocol for elucidating physiologic substrates of little characterized or uncharacterized PKMTs

2020-07-02 | PXD019886 | Pride

Phosphoproteomics of Arabidopsis flg22 treated nuclear samples

Project description:To study the effect of microbe-associated molecular pattern (MAMP) treatment (flg22) on the phosphorylation of nuclear proteins, we treated Arabidopsis plants with flg22 and after isolation of nuclear proteins, we enriched for phosphopeptides and then carried out LC-MS/MS analyses. We used statistical analysis tools to identify differentially phosphorylated proteins in response to MAMP treatment in the mock and treated samples.

2022-02-28 | PXD008403 | Pride

PglL enzymes of Burkholderia species are serine preferring oligosaccharidetransferases which targets conserved proteins and sites across the genus -DsbA1 analysis

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2021-07-21 | PXD023955 | Pride

Genome-wide consequences of compromised NMD and their relavence for variable clinical phenotype of patients with UPF3B mutations [CNV]

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2012-04-30 | E-GEOD-37672 | biostudies-arrayexpress

PglL enzymes of Burkholderia species are serine preferring oligosaccharidetransferases which targets conserved proteins and sites across the genus -DsbA1 analysis of Burkholderia humptydooensis MSMB43 and Burkholderia ubonensis MSMB22

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2021-07-21 | PXD024056 | Pride

Genome-wide consequences of compromised NMD and their relavence for variable clinical phenotype of patients with UPF3B mutations

Project description:This SuperSeries is composed of the following subset Series: GSE27125: Genome-wide consequences of compromised NMD and their relavence for variable clinical phenotype of patients with UPF3B mutations [mRNA profiling] GSE27199: Genome-wide consequences of compromised NMD and their relavence for variable clinical phenotype of patients with UPF3B mutations [RNA-seq] GSE27412: Genome-wide consequences of compromised NMD and their relavence for variable clinical phenotype of patients with UPF3B mutations [CNV] Refer to individual Series

2011-11-01 | E-GEOD-27433 | biostudies-arrayexpress

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