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Role of Chd8 in Xist regulation

ABSTRACT: In this project, we have studied the role of Chd8 in Xist regulation and XCI initiation by means of Chd8 Knock-Downs (KD) and Knock-Out (KO).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Embryonic Stem Cell

SUBMITTER: Frank Stein

LAB HEAD: Andrea Cerase

PROVIDER: PXD024155 | Pride | 2021-09-10

REPOSITORIES: Pride

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Chd8 regulates X chromosome inactivation in mouse through fine-tuning control of Xist expression.

Cerase Andrea A Young Alexander N AN Ruiz Nerea Blanes NB Buness Andreas A Sant Gabrielle M GM Arnold Mirjam M Di Giacomo Monica M Ascolani Michela M Kumar Manish M Hierholzer Andreas A Trigiante Giuseppe G Marzi Sarah J SJ Avner Philip P

Communications biology 20210415 1

Female mammals achieve dosage compensation by inactivating one of their two X chromosomes during development, a process entirely dependent on Xist, an X-linked long non-coding RNA (lncRNA). At the onset of X chromosome inactivation (XCI), Xist is up-regulated and spreads along the future inactive X chromosome. Contextually, it recruits repressive histone and DNA modifiers that transcriptionally silence the X chromosome. Xist regulation is tightly coupled to differentiation and its expression is ...[more]

PMID: 33859315

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