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Identification of indisulam-mediated DCAF15 interacting proteins by TurboID and shotgun proteomics

ABSTRACT: In this project, we used proximity labeling with TurboID to identify proteins that interact with DCAF15 in the presence of indisulam in a gastric cancer AGS cell line.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Gastric Cancer Cell Line

SUBMITTER: jiaqi lu

LAB HEAD: Guoqiang Xu

PROVIDER: PXD024168 | Pride | 2021-08-26

REPOSITORIES: Pride

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Proximity Labeling, Quantitative Proteomics, and Biochemical Studies Revealed the Molecular Mechanism for the Inhibitory Effect of Indisulam on the Proliferation of Gastric Cancer Cells.

Lu Jiaqi J Jiang Honglv H Li Dan D Chen Tao T Wang Yuhong Y Pu Zhongjian Z Xu Guoqiang G

Journal of proteome research 20210822 9

Indisulam exhibits antitumor activity against several cancer cells. Although the DCAF15-indisulam-RBM39 axis has been well documented in the inhibition of cancer cell growth, it is unknown whether RBM39 degradation alone is the mechanism of action of indisulam. Here, we verified the inhibitory effect of indisulam on the proliferation of gastric cancer cells and its dependence on DCAF15. Proximity-dependent biotin labeling with TurboID and quantitative proteomics revealed that indisulam indeed pr ...[more]

PMID: 34420308

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