Comparision of Nano and Micro-flow LC-MS/MS Based Single Shot Proteomics

ABSTRACT: In this study, we systematically optimized the micro-flow LC-MS/MS system based ssDDA proteomics. Our results show that combination of 2 – 3 h gradient with prosit rescore method identified the highest number of proteins and peptides. There is no benefit to increase column length. A single 3 h run identified about 9,200 proteins from 50 µg of Arabidopsis protein digest. The micro-flow LC-MS/MS system based ssDDA proteomics can be widely used for routine proteomics analysis of large cohort samples. We compared our micro data with the state of art nano LC data.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Yangyang Bian  

LAB HEAD: Bernhard Kuster

PROVIDER: PXD024169 | Pride | 2021-07-01

REPOSITORIES: Pride