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Mia3 enables efficient secretion by constraining COPII vesicle budding - secreted proteome

ABSTRACT: Analysis of secreted proteome from cells engineered with knockout of Mia3 gene isoforms encoding TANGO1L and TANGO1S

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

SUBMITTER: David Stephens

LAB HEAD: David Stephens

PROVIDER: PXD024221 | Pride | 2021-08-25

REPOSITORIES: Pride

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Publications

A general role for TANGO1, encoded by MIA3, in secretory pathway organization and function.

McCaughey Janine J Stevenson Nicola L NL Mantell Judith M JM Neal Chris R CR Paterson Alex A Heesom Kate K Stephens David J DJ

Journal of cell science 20210907 17

Complex machinery is required to drive secretory cargo export from the endoplasmic reticulum (ER), which is an essential process in eukaryotic cells. In vertebrates, the MIA3 gene encodes two major forms of transport and Golgi organization protein 1 (TANGO1S and TANGO1L), which have previously been implicated in selective trafficking of procollagen. Using genome engineering of human cells, light microscopy, secretion assays, genomics and proteomics, we show that disruption of the longer form, TA ...[more]

PMID: 34350936

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