Ontology highlight
ABSTRACT:
INSTRUMENT(S): LTQ Orbitrap
ORGANISM(S): Homo Sapiens (human)
TISSUE(S): Epithelial Cell
SUBMITTER: Sheena Wee
LAB HEAD: Jayantha Gunaratne
PROVIDER: PXD024464 | Pride | 2021-11-30
REPOSITORIES: Pride
Baskaran Yohendran Y Tay Felicia Pei-Ling FP Ng Elsa Yuen Wai EYW Swa Claire Lee Foon CLF Wee Sheena S Gunaratne Jayantha J Manser Edward E
Nature communications 20210907 1
Human PAK4 is an ubiquitously expressed p21-activated kinase which acts downstream of Cdc42. Since PAK4 is enriched in cell-cell junctions, we probed the local protein environment around the kinase with a view to understanding its location and substrates. We report that U2OS cells expressing PAK4-BirA-GFP identify a subset of 27 PAK4-proximal proteins that are primarily cell-cell junction components. Afadin/AF6 showed the highest relative biotin labelling and links to the nectin family of homoph ...[more]