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Nsp1 and Nsp2 interactome and proteome

ABSTRACT: The samples represent pulldowns of protein Nsp1 and Nsp2 from SARS-CoV and SARS-CoV2, as well its unfunctional mutants. Additionally, total proteomes of corresponding samples were analyzed.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Olga Shevchuk

LAB HEAD: Prof. Dr. Albert Sickmann

PROVIDER: PXD024480 | Pride | 2022-05-10

REPOSITORIES: Pride

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Publications

The key features of SARS-CoV-2 leader and NSP1 required for viral escape of NSP1-mediated repression.

Bujanic Lucija L Shevchuk Olga O von Kügelgen Nicolai N Kalinina Anna A Ludwik Katarzyna K Koppstein David D Zerna Nadja N Sickmann Albert A Chekulaeva Marina M

RNA (New York, N.Y.) 20220301 5

SARS-CoV-2, responsible for the ongoing global pandemic, must overcome a conundrum faced by all viruses. To achieve its own replication and spread, it simultaneously depends on and subverts cellular mechanisms. At the early stage of infection, SARS-CoV-2 expresses the viral nonstructural protein 1 (NSP1), which inhibits host translation by blocking the mRNA entry tunnel on the ribosome; this interferes with the binding of cellular mRNAs to the ribosome. Viral mRNAs, on the other hand, overcome t ...[more]

PMID: 35232816

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