Dataset Information

Large-scale analysis of the androgenic gland proteome in the Chinese mitten crab Eriocheir sinensis

ABSTRACT: Large-scale analysis of the androgenic gland proteome in the Chinese mitten crab Eriocheir sinensis

INSTRUMENT(S):

ORGANISM(S): Eriocheir Sinensis (chinese Mitten Crab)

SUBMITTER: Chenchang Bao

LAB HEAD: Chenchang Bao

PROVIDER: PXD024496 | Pride | 2021-09-10

REPOSITORIES: Pride

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Publications

The Chinese mitten crab genome provides insights into adaptive plasticity and developmental regulation.

Cui Zhaoxia Z Liu Yuan Y Yuan Jianbo J Zhang Xiaojun X Ventura Tomer T Ma Ka Yan KY Sun Shuai S Song Chengwen C Zhan Dongliang D Yang Yanan Y Liu Hourong H Fan Guangyi G Cai Qingle Q Du Jing J Qin Jing J Shi Chengcheng C Hao Shijie S Fitzgibbon Quinn P QP Smith Gregory G GG Xiang Jianhai J Chan Tin-Yam TY Hui Min M Bao Chenchang C Li Fuhua F Chu Ka Hou KH

Nature communications 20210422 1

The infraorder Brachyura (true or short-tailed crabs) represents a successful group of marine invertebrates yet with limited genomic resources. Here we report a chromosome-anchored reference genome and transcriptomes of the Chinese mitten crab Eriocheir sinensis, a catadromous crab and invasive species with wide environmental tolerance, strong osmoregulatory capacity and high fertility. We show the expansion of specific gene families in the crab, including F-ATPase, which enhances our knowledge ...[more]

PMID: 33888695

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Project description:Spiroplasma eriocheiris, has been identified as a novel lethal pathogen of Eriocheir sinensis tremor disease (TD), one neurological disease with typically paroxysmal tremors of the pereiopod. This pathogen infected and multiplied in the hemocytes of E. sinensis as the first target cells, and then follow the blood circulation to infect the crab other tissues. S. eriocheiris infected the nerves tissue is the directly reason of TD. But the pathogenic mechanism of TD was still few known. Firstly, in the current study, the phosphoproteomic changes of E. sinensis thoracic ganglion after S. eriocheiris infection were obtained. KEGG pathway analysis show Wnt signaling pathways was restrained, corresponding many nervous system development and signal transmission pathway also destroyed. Base on the identified modified sequence, several peptides (GSK3β, SYN, VAMP and SNAMP-25) were selected to synthesize chemically and prepare phosphorylated antibodies. The differentially expressed phosphorylated proteins GSK3β-pSer9, VAMP-pSer72, SNAP25-pSer102, and SYN-pSer134 in thoracic ganglion were significantly down-regulated verified by immunohistochemistry and western blot, this results are similar with the phosphoproteomic. By qRT-PCR, western blot, RNA interference and inhibitor experiments, when the S. eriocheiris infected the hemocytes of crab, the GSK-3β and β-Catenin in Wnt-β-Catenin pathway were restrained similar in the thoracic ganglion. The S. eriocheiris can restricts the hemocytes Wnt-β-Catenin pathway to help itself infection no matter in vivo or in vitro. Neurotransmitter metabolite analysis showed that four kinds of neurotransmitters (5-Hydroxy-L-tryptophan, Serotonin, Acetylcholine and γ-Amino-butyric acid) in thoracic ganglion were metabolic disorders in E. sinensis thoracic ganglion after S. eriocheiris infection. The present work could serve as a basis for understanding the role of Wnt signaling pathway in the process of S. eriocheiris invasion E. sinensis hemocytes and causing paroxysmal tremors of E. sinensis the pereiopod.

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