The resistance of oilseed rape microspore-derived embryos to osmotic stress is associated with the accumulation of proteins in energy metabolism, redox homeostasis and up-regulation of abscisic acid and cytokinin contents

ABSTRACT: The aim of the present study was to investigate the response of rapeseed microspore-derived embryos (MDE) to osmotic stress at proteome level. The PEG-induced osmotic stress was studied in cotyledonary MDE of two genotypes: Cadeli (D), and Viking (V), which were previously reported to reveal contrasting leaf proteome responses under drought. 156 representative protein spots – obtained by 2D-DIGE – have been selected for MALDI-TOF/TOF, 63 proteins have been successfully identified and divided into 8 functional groups. Eight selected protein accumulation trends were validated by using qRT-PCR. Biomass accumulation in treated D was significantly higher (3-fold) than in V, so we can claim D as resistant to osmotic stress. Cultivar D displayed resistance strategy thanks to accumulation of proteins in energy metabolism, redox homeostasis, protein destination and signalling functional groups accompanied by high ABA and active cytokinins. In contrast, V protein profile displayed high requirements of energy (ATP) and nutrients with a significant number of stress-related proteins and cell structure changes accompanied by quick down-regulation for active CK content, SA, JA, GA20. MDE proteome profile is discussed together with leaf proteome results from our previous study (Urban et al., 2017, 30:152, J. Proteomics). We propose different mechanisms to cope with osmotic stress in the genotypes studied. This proteomic study is a first step to validate MDE as a suitable model for follow-up research on characterization of new crossings, and can be used for selection of resistant genotypes.

INSTRUMENT(S): TOF/TOF 5800

ORGANISM(S): Brassica Napus (rape)

TISSUE(S): Microspore

SUBMITTER: Sebastien Planchon  

LAB HEAD: Mgr. Pavel Vitamvas, PhD

PROVIDER: PXD024552 | Pride | 2021-06-14

REPOSITORIES: Pride