SAPT, a fast and efficient approach for simultaneous profiling of protein N- and C-terminome

ABSTRACT: Protein termini play pivotal roles in various biological processes. Although a series of terminomic strategies have been proposed for the analysis of protein N-termini or protein C-termini separately, few can analyze both ends of proteins at the same time. Herein, we developed a workflow, termed Simultaneous Analysis of Protein N- and C- Terminome (SAPT) based on strong cation exchange chromatography (SCX) to study protein terminome simultaneously.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: zhongjie wang  

LAB HEAD: Haojie Lu

PROVIDER: PXD024573 | Pride | 2021-07-29

REPOSITORIES: Pride