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Too Hot to Handle: Antibacterial Peptides Identified in Ghost Pepper

ABSTRACT: Capsicum spp. (hot peppers) demonstrate a range of interesting bioactive properties spanning anti-inflammatory, antioxidant, and antimicrobial activities. While several species within the genus are known to produce antimicrobial peptides (AMPs), AMP sequence mining of genomic data indicates this space remains largely unexplored. Herein, in silico AMP predictions are paired with peptidomics to identify novel AMPs from the interspecific hybrid ghost pepper (Capsicum chinense x frutescens). AMP prediction algorithms reveal 115 putative AMPs within the Capsicum chinense genome of which 14 were identified in the aerial tissue peptidome. PepSAVI-MS, de novo sequencing, and complementary approaches were used to fully molecularly characterize two novel AMPs, CC-AMP1 and CC-AMP2, including elucidation of post-translational modifications and disulfide bond connectivity. Both CC-AMP1 and CC-AMP2 have little homology with known AMPs and exhibit low µM antimicrobial activity against gram-negative bacteria including Escherichia coli and Klebsiella pneumoniae. These findings demonstrate the complementary nature of peptidomics, bioactivity-guided discovery, and bioinformatics-based investigations to more fully characterize plant AMP profiles.

INSTRUMENT(S): TripleTOF 5600, Q Exactive HF

ORGANISM(S): Capsicum Chinense X Capsicum Frutescens

TISSUE(S): Leaf

SUBMITTER: Leslie Hicks

LAB HEAD: Leslie M. Hicks

PROVIDER: PXD024605 | Pride | 2021-08-13

REPOSITORIES: Pride

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