Dataset Information

Proteomic analysis of Caenorhabditis elegans wound model reveals novel molecular players involved in repair

ABSTRACT: Now a days, wound healing is becoming a global threat that impact economy of the country severely. Though the steps involved in wound healing are well characterised, direct therapeutics for the accelerated wound healing is comparatively less. This is solely because of the incomplete mechanism of healing and the lack of knowledge on molecular players involved in wound healing. Hence, in the present study, we have investigated the molecular players involved in wound healing process using a versatile model organism Caenorhabditis elegans through proteomic analyses. Especially, we have employed the high through put proteomic analyses tools such as 2-D GE and LCMS/MS analysis to uncover the molecular players involved in wound healing. As a result of 2-D GE analysis, a total of 35 differentially regulated proteins were identified during injury in which 22 and 13 proteins were upregulated and downregulated, respectively. Bioinformatics analyses result suggested that the upregulated proteins have crucial roles in serotonin/ acetyl choline synthesis and calcium signalling, whereas downregulated proteins were majorly involving in ubiquitin mediated proteolysis. Additionally, the mRNA levels of up and downregulated proteins were validated using qPCR analysis. Overall, the study suggested that injury initiates multiple molecular mechanisms to repair the damage.

INSTRUMENT(S): AXIMA Performance MALDI-TOF/TOF

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Whole Body

DISEASE(S): Wounds And Injuries

SUBMITTER: Krishnaswamy Balamurugan

LAB HEAD: Dr. KB Lab (Host Pathogen Interaction lab)

PROVIDER: PXD024629 | Pride | 2021-04-16

REPOSITORIES: Pride

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Proteomic analysis of Caenorhabditis elegans wound model reveals novel molecular players involved in repair.

Pooranachithra Murugesan M Satheesh Kumar Chelladurai C Bhaskar James Prabhanand JP Venkateswaran Krishnan K Ravichandiran Velayutham V Balamurugan Krishnaswamy K

Journal of proteomics 20210405

Wound repair is a multistep process which involves coordination of multiple molecular players from different cell types and pathways. Though the cellular processes that are taking place in order to repair damage is already known, molecular players involved in crucial pathways are still scarce. In this regard, the present study intends to uncover crucial players that are involved in the central repair events through proteomics approach which included 2-D GE and LC-MS/MS using Caenorhabditis elega ...[more]

PMID: 33831597

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Dataset Information

Proteomic analysis of Caenorhabditis elegans wound model reveals novel molecular players involved in repair

Publications

Proteomic analysis of Caenorhabditis elegans wound model reveals novel molecular players involved in repair.

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